Condition: Used

In excellent condition, tested and working. Sourced from a lab closure. Manufactured 2022. Express worlwide shipping.

Supplied with a power adapter, ethernet and USB with software.

Harness the power of Illumina next-generation sequencing (NGS) technology in an easy-to-use, inexpensive ecosystem right in your lab

Highly accurate data, fast run times, and a maximum output of 1.2 Gb in a simple, compact benchtop sequencing system

The Illumina iSeq 100 is a compact and affordable benchtop sequencer designed for smaller labs or targeted sequencing applications. It fills a specific niche in the next-generation sequencing (NGS) market by providing fast, accessible, and high-quality data for projects that don't require the throughput of larger production-scale sequencers. The iSeq 100 maintains the accuracy and reliability associated with Illumina's sequencing by synthesis (SBS) chemistry.

Key Innovations

CMOS Sensor Technology: The iSeq 100 sets itself apart by utilizing a complementary metal-oxide semiconductor (CMOS) image sensor chip, similar to those found in smartphone cameras. This chip is paired with a specialized one-channel SBS chemistry designed for optimal signal detection. By replacing conventional laser optics and camera systems, the CMOS sensor design simplifies the instrument significantly, reduces instrument cost and results in a smaller footprint, while still delivering accurate data.

Simplified Workflow: The iSeq 100 boasts a streamlined setup and operation process. Prefilled reagent cartridges and an intuitive software interface minimize hands-on time, making NGS accessible to labs without dedicated sequencing specialists.

Features and Technologies

Sequencing by Synthesis (SBS): The iSeq 100 employs Illumina's proven SBS chemistry, using fluorescently labeled nucleotides to generate highly accurate sequencing data.

Single-Lane Flow Cell: The iSeq 100's flow cell has a single sequencing lane, optimized for smaller output runs.

Data Output: Capable of producing up to 1.2 Gb of data per run.

Run Time: Sequencing runs can be completed in 19 hours or less.

Read Length: Supports up to 2 x 150 bp (paired-end) reads.

Intended Uses and Specific Applications

The iSeq 100's capabilities make it ideal for various research applications, including:

Small Whole-Genome Sequencing: Sequencing of smaller genomes like bacteria, viruses, and plasmids. Suitable library prep kits include Illumina DNA Prep Library Prep Kit or Nextera XT DNA Library Prep Kit.

Targeted Gene Sequencing: Analysis of specific genes or regions of interest (panels). Suitable kits include TruSight One Panels or AmpliSeq Custom Panels.

Gene Expression Profiling: Measuring RNA expression using kits like Illumina Stranded mRNA Prep.

16S Metagenomics: Characterization of microbial communities. Suitable library prep includes Illumina DNA Prep Library Prep Kit or the Illumina 16S Metagenomic Sequencing Protocol.

Comparison with Competing Instruments

Illumina MiniSeq: The MiniSeq offers higher throughput (up to 7.5 Gb per run). It's a better choice for projects requiring larger sequencing output, but it comes with increased instrument cost and complexity.

Complete Genomics DNBSEQ-E25: This platform offer higher max output and similar speeds, but it cannot offer the shorter sequencing read lengths available to the iSeq 100 and may come at a higher upfront cost.

Special Considerations

The iSeq 100 is a compelling option when these factors are important:

Ease of Use: Ideal for labs that want reliable NGS data without the complexity or in-house expertise requirements of larger sequencing platforms.

Affordability: Offers a lower initial investment compared to larger Illumina sequencers or comparable competitors.

Fast Turnaround Time: Delivers results quickly when time is a critical factor.

Sequencing by synthesis chemistry

The iSeq 100 System harnesses proven Illumina sequencing by synthesis (SBS) chemistry to deliver highly accurate data and robust performance for a broad range of applications. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.

One-channel SBS chemistryThe iSeq 100 System combines Illumina SBS chemistry with complementary metal-oxide semiconductor (CMOS) technology to deliver one-channel SBS chemistry. One-channel SBS chemistry on a CMOS chip helps to reduce instrument cost and allows for a smaller benchtop footprint, all while delivering accurate data.

Datasheet

Shipping & Returns Policy

Shipping Policy

• Processing Time: Orders are typically processed and dispatched within 1 business day.
• Shipping Methods: We offer Expedited Shipping to all destinations using UPS/DHL/Fedex/Royal Mail and other reputable carriers.
• Shipping Times: Mainland UK: 1-2 Business Days / Mainland Europe and USA: 2-3 Business Days / Rest of the World 5-7 Business Days.
• Shipping Costs: These are calculated at checkout based on the shipping method, destination and package dimensions.
• CUSTOMS AND TAXES: It is YOUR responsibility to liaise with customs in your country and ensure your package is cleared and any taxes are paid.
• Shipping Destinations: We ship worldwide.
• Tracking: Once your order is shipped, we will provide you with a tracking number via email.
• Insurance: All shipments are insured. In case of damage, please contact us via email within 48 hours of receiving the product with photographic evidence, please also ensure a report is logged with the delivery driver.

Returns Policy

• Return Period: In the event of an item being received which is 'not as specified', you can return your purchase within 14 days of delivery.
• Condition of Items: Items must be returned unused and in their original packaging.
• Return Method: All returns must be notified in writing to hello@ttinstruments.com, the email should accompany the order number, date and reason for return and must be within 14 days of delivery.
• Restocking Fees: A restocking fee of up-to 20% is applicable for items returned for any reason other than 'not as specified'.
• Refund Method: Refunds are processed to the original method of payment within 5 business days of receiving the return.
• Exchanges: We do not offer exchanges. It will either be a replacement or a refund.
• Return Shipping Costs: Return shipping costs will be borne by the customer unless the item is 'not as specified', in this case we will arrange for the return by way of a prepaid shipping label.